• toxoplasma gondii cyst

Toxoplasma ELISA Reagent Line
Catalog# Toxoplasma ELISA


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Toxoplasmosis is an economically and medically important zoonotic disease which is caused by the protozoan intracellular parasite, Toxoplasma gondii. A wide range of terrestrial and aquatic vertebrate hosts are susceptible to infection. T. gondii infection is common in humans and animals, including livestock and wildlife. Although infection in most immunocompetent hosts results in mild or undetectable illness, serious clinical disease can occur. Transmission occurs by the consumption of tissues of infected animals, and the ingestion of fresh fruits and vegetables contaminated with oocysts shed with the feces of recently infected cats. Congenital transmission from mother to foetus can also occur. Several serological methods have been developed for the detection of antibodies to T. gondii in animals and humans. The indirect ELISA is the most commonly used assay with advantages of speed, reduced costs, high through-put, objective readings and the potential for high sensitivity and specificity. The major disadvantage of this assay has been in the use host specific anti-immunoglobulin reagents which limit the use of the assay to individual host species. The multi-host species assay presented here as a kit removes this restriction by using protein A/G. This multi-host species ELISA has been developed and validated for use on serum and other body fluid samples on a wide range of mammalian domestic and wildlife hosts. Comparative studies show that it is highly specific and sensitive and equivalent in performance to traditional ELISA methods and agglutination assays such as the MAT which is also available as a kit at New Life Diagnostics. Comparative analysis of various serological assays for the detection of T. gondii infection in various species of terrestrial and aquatic mammals has shown that the performance of the ELISA-A/G was similar to those of ELISA-IgG, MAT and Western Blot. Compared to the ELISA-IgG, the relative sensitivity and specificity of the ELISA-A/G were 90% and 100%, respectively.


References


Al-Adhami, B.H., Simard, M., Hernández-Ortiz, A., Boireau, C., Gajadhar, A.A. 2016. Development and evaluation of a modified agglutination test for diagnosis of Toxoplasma infection using tachyzoites cultivated in cell culture. Food and Waterborne Parasitol. doi: 10.1016/j.fawpar.2015.12.001.


Al-Adhami, B.H., Gajadhar, A.A. 2014. A new multi-host species indirect ELISA using protein A/G conjugate for detection of anti-Toxoplasma gondii IgG antibodies with comparison to ELISA-IgG, agglutination assay and Western Blot. Vet. Parasitol. 200: 66-73.


Dubey, J.P. 2010. Toxoplasmosis of animals and humans. 2nd Edition. CRC Press. Gajadhar, A.A. 2014. Foodborne parasites in the food supply web: Occurrence and control. 1st Edition. Woodhead/Elsevier Publishing.


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