Toxoplasma MAT Microwell Reagent Line Catalog# Toxoplasma MAT
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Toxoplasmosis is an economically and medically important zoonotic disease which is caused by the protozoan intracellular parasite, Toxoplasma gondii. A wide range of terrestrial and aquatic vertebrate hosts are susceptible to infection. T. gondii infection is common in humans and animals, including livestock and wildlife. Although infection in most immunocompetent hosts results in mild or undetectable illness, serious clinical disease can occur. Transmission occurs by the consumption of tissues of infected animals, and the ingestion of fresh fruits and vegetables contaminated with oocysts shed with the feces of recently infected cats. Congenital transmission from mother to foetus can also occur. Several serological methods have been developed for the detection of antibodies to T. gondii in animals and humans. Most antibody-detection methods are relatively complex, require specialized laboratory equipment and limited by the need for host species specific conjugates. The Modified Agglutination Test (MAT) is a direct agglutination assay which is rapid and simple to perform without the use of complex laboratory facilities. It uses T. gondii tachyzoites fixed in formalin to detect anti-IgG antibodies in blood, serum, and other fluids from the body, such as meat juice. The MAT has a sensitivity of 83% and a specificity of 90% and has been applied successfully on samples from humans and a wide variety of animals, including dogs, cats, pigs, cattle, sheep, goats, poultry, deer, elk, caribou, bears, seals, etc. In very early infection when IgM, but not IgG is present false negative results can occur. This may be managed by following up with an IgM specific assay such as IgM-IFA or IgG-ELISA, or by testing a second sample a few weeks later.